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Coding
Part:BBa_K3279003
Designed by: Shujie Liao Group: iGEM19_CAU_China (2019-10-14)
lycopene beta-cyclase (CrtY)
This part is a lycopene beta-cyclase which transforms lycopene intoβ-carotene. The enzyme is a non-redox flavoprotein, containing FADH2 that is used for stabilization of a transition state. Lycopene has a psi-end group at both ends. When acting on one end, the enzyme forms gamma-carotene. When acting on both ends it forms beta-carotene. It also acts on neurosporene to give beta-zeacarotene. This sequence is optimized for use in E.coli.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 572
Illegal PstI site found at 771 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 572
Illegal PstI site found at 771 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 153
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 572
Illegal PstI site found at 771 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 572
Illegal PstI site found at 771 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 877
Illegal SapI.rc site found at 700
[edit]
Categories
Parameters
None |