Coding

Part:BBa_K3279003

Designed by: Shujie Liao   Group: iGEM19_CAU_China   (2019-10-14)


lycopene beta-cyclase (CrtY)

This part is a lycopene beta-cyclase which transforms lycopene intoβ-carotene. The enzyme is a non-redox flavoprotein, containing FADH2 that is used for stabilization of a transition state. Lycopene has a psi-end group at both ends. When acting on one end, the enzyme forms gamma-carotene. When acting on both ends it forms beta-carotene. It also acts on neurosporene to give beta-zeacarotene. This sequence is optimized for use in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 572
    Illegal PstI site found at 771
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 572
    Illegal PstI site found at 771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 572
    Illegal PstI site found at 771
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 572
    Illegal PstI site found at 771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 877
    Illegal SapI.rc site found at 700


[edit]
Categories
Parameters
None